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Research Methods

Our laboratory facilities allow both students and faculty to pursue their research interests in a variety of ways. Among the methods currently being employed are:

  • Transgenic Mice
  • Biosensors
  • Caged Second Messengers
  • cDNA and Genomic Cloning
  • Computerized Reconstruction Techniques
  • Electrophysiological Recording of Synaptic Connections
  • Enzyme Assays, Including Phosphorylation Assays
  • Expression of Recombinant Proteins (including transfection with viral vectors and gene guns)
  • Fluorescent Indicator Measurements of Intracellular Calcium and pH
  • Gene Microarrays
  • HPLC
  • Immunocytochemistry
  • Measurement of Cellular Proteins by Immunobiological Assays, Including RIA, ELISA, and Western Blots
  • mRNA measurement by RNase Protection Assay, Northern Blots and Quantitative Competitive PCR
  • Neuroanatomical Pathway Tracing
  • Polymerase Chain Reaction (PCR)
  • Primary Cell Culture
  • Promoter Analysis of Control of Gene Expression (including reporter assays and EMSAs)
  • Protein Purification
  • Proteomics: 2-D Gels and Mass Spectrometry
  • Receptor-binding Assays and Drug-receptor Interactions
  • Single-channel Recording
  • Yeast Two-hybrid Screens
  • X-ray Crystallography