Research Methods

Our laboratory facilities allow both students and faculty to pursue their research interests in a variety of ways. Among the methods currently being employed are:

• Transgenic Mice
• Biosensors
• Caged Second Messengers
• cDNA and Genomic Cloning
• Computerized Reconstruction Techniques
• Electrophysiological Recording of Synaptic Connections
• Enzyme Assays, Including Phosphorylation Assays
• Expression of Recombinant Proteins (including transfection with viral vectors and gene guns)
• Fluorescent Indicator Measurements of Intracellular Calcium and pH
• Gene Microarrays
• HPLC
• Immunocytochemistry
• Measurement of Cellular Proteins by Immunobiological Assays, Including RIA, ELISA, and Western Blots
• mRNA measurement by RNase Protection Assay, Northern Blots and Quantitative Competitive PCR
• Neuroanatomical Pathway Tracing
• Polymerase Chain Reaction (PCR)
• Primary Cell Culture
• Promoter Analysis of Control of Gene Expression (including reporter assays and EMSAs)
• Protein Purification
• Proteomics: 2-D Gels and Mass Spectrometry
• Receptor-binding Assays and Drug-receptor Interactions
• Single-channel Recording
• Yeast Two-hybrid Screens
• X-ray Crystallography

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